puc19 plasmid backbone (TaKaRa)
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TaKaRa
puc19 plasmid backbone
Puc19 Plasmid Backbone, supplied by TaKaRa, used in various techniques. Bioz Stars score: 98/100, based on 1078 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/puc19+plasmid+backbone/In-Fusion+Snap+Assembly+Master+Mix/pm41565826-538-25-28
Average 98 stars, based on 1078 article reviews
Puc19 Plasmid Backbone, supplied by TaKaRa, used in various techniques. Bioz Stars score: 98/100, based on 1078 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/puc19+plasmid+backbone/In-Fusion+Snap+Assembly+Master+Mix/pm41565826-538-25-28
Average 98 stars, based on 1078 article reviews
puc19 plasmid backbone - by Bioz Stars,
2026-10
98/100 stars
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other:Article Title: Critical role for a high-plasticity cell state in lung cancer Article Snippet: The diagram in e was created using BioRender. Plasmid Preparation:Article Title: Functional interrogation uncovers a critical role for a high-plasticity cell state in lung adenocarcinoma Article Snippet: For the generation of Slc4a11-FSF-MCD donor vector, homology arms ∼1200 bp in length 5’ and 3’ to the end of Slc4a11 exon 21 ( Extended Data Fig. 1b ) were amplified from genomic DNA of C57BL/6 mouse embryonic stem cell (mESC) using high-fidelity PCR (NEB, #M0494). .. A homology-directed repair template donor vector was constructed by flanking frt-bGlobinpA-(PGK-Hygromycin-pA)i-frt-P2A-mScarlet-T2A-CreERT2-P2A-DTR-WPRE-bGHpA cassette with the 5’ and 3’ homology arms and cloned into the Article Title: Alveolar differentiation drives resistance to KRAS inhibition in lung adenocarcinoma Article Snippet: Homology arms ∼1500 bp in length 5’ and 3’ to the end of Hopx exon 3 were amplified from C57/BL6 genomic DNA using high-fidelity PCR (KME-101, Toyobo). .. A homology-directed repair template donor vector where frt-PGK-Hygro-pA-frt-T2A-mScarlet-Akaluc-T2A-CreER-P2A-DTR is flanked by the homology arms was cloned into the Article Title: Critical role for a high-plasticity cell state in lung cancer. Article Snippet: Generation of donor vectors for embryonic stem cell targeting For the generation of the Slc4a11-FSF-MCD donor vector, homology arms of around 1,200 bp in length 5′ and 3′ to the end of Slc4a11 exon 21 (Extended Data Fig. 1b) were amplified from genomic DNA of C57BL/6 mES cells using high-fidelity PCR (NEB, M0494). .. A homology-directed repair template donor vector was constructed by flanking the frt-bGlobinpA-(PGK-Hygromycin-pA)i-frt-P2A-mScarlet-T2A-CreER T2-P2A-DTR-WPRE-bGHpA cassette with the 5′ and 3′ homology arms and cloned into the Construct:Article Title: Functional interrogation uncovers a critical role for a high-plasticity cell state in lung adenocarcinoma Article Snippet: For the generation of Slc4a11-FSF-MCD donor vector, homology arms ∼1200 bp in length 5’ and 3’ to the end of Slc4a11 exon 21 ( Extended Data Fig. 1b ) were amplified from genomic DNA of C57BL/6 mouse embryonic stem cell (mESC) using high-fidelity PCR (NEB, #M0494). .. A homology-directed repair template donor vector was constructed by flanking frt-bGlobinpA-(PGK-Hygromycin-pA)i-frt-P2A-mScarlet-T2A-CreERT2-P2A-DTR-WPRE-bGHpA cassette with the 5’ and 3’ homology arms and cloned into the Article Title: Critical role for a high-plasticity cell state in lung cancer. Article Snippet: Generation of donor vectors for embryonic stem cell targeting For the generation of the Slc4a11-FSF-MCD donor vector, homology arms of around 1,200 bp in length 5′ and 3′ to the end of Slc4a11 exon 21 (Extended Data Fig. 1b) were amplified from genomic DNA of C57BL/6 mES cells using high-fidelity PCR (NEB, M0494). .. A homology-directed repair template donor vector was constructed by flanking the frt-bGlobinpA-(PGK-Hygromycin-pA)i-frt-P2A-mScarlet-T2A-CreER T2-P2A-DTR-WPRE-bGHpA cassette with the 5′ and 3′ homology arms and cloned into the Clone Assay:Article Title: Functional interrogation uncovers a critical role for a high-plasticity cell state in lung adenocarcinoma Article Snippet: For the generation of Slc4a11-FSF-MCD donor vector, homology arms ∼1200 bp in length 5’ and 3’ to the end of Slc4a11 exon 21 ( Extended Data Fig. 1b ) were amplified from genomic DNA of C57BL/6 mouse embryonic stem cell (mESC) using high-fidelity PCR (NEB, #M0494). .. A homology-directed repair template donor vector was constructed by flanking frt-bGlobinpA-(PGK-Hygromycin-pA)i-frt-P2A-mScarlet-T2A-CreERT2-P2A-DTR-WPRE-bGHpA cassette with the 5’ and 3’ homology arms and cloned into the Article Title: Alveolar differentiation drives resistance to KRAS inhibition in lung adenocarcinoma Article Snippet: Homology arms ∼1500 bp in length 5’ and 3’ to the end of Hopx exon 3 were amplified from C57/BL6 genomic DNA using high-fidelity PCR (KME-101, Toyobo). .. A homology-directed repair template donor vector where frt-PGK-Hygro-pA-frt-T2A-mScarlet-Akaluc-T2A-CreER-P2A-DTR is flanked by the homology arms was cloned into the Article Title: Critical role for a high-plasticity cell state in lung cancer. Article Snippet: Generation of donor vectors for embryonic stem cell targeting For the generation of the Slc4a11-FSF-MCD donor vector, homology arms of around 1,200 bp in length 5′ and 3′ to the end of Slc4a11 exon 21 (Extended Data Fig. 1b) were amplified from genomic DNA of C57BL/6 mES cells using high-fidelity PCR (NEB, M0494). .. A homology-directed repair template donor vector was constructed by flanking the frt-bGlobinpA-(PGK-Hygromycin-pA)i-frt-P2A-mScarlet-T2A-CreER T2-P2A-DTR-WPRE-bGHpA cassette with the 5′ and 3′ homology arms and cloned into the |